Transcription Factor Loading on the MMTV Promoter: A Bimodal Mechanism for Promoter Activation
Abstract
The mouse mammary tumor virus (MMTV) promoter attains a phased array of six nucleosomes when introduced into rodent cells. This architecture excludes nuclear factor 1/CCAAT transcription factor (NF1/CTF) from the promoter before glucocorticoid treatment and hormone-dependent access of nucleolytic agents to promoter DNA. In contrast, when the promoter was transiently introduced into cells, NF1/CTF was bound constitutively and nucleolytic attack was hormone-independent. Thus, induction at this promoter was a bimodal process involving receptor-dependent remodeling of chromatin that allows NF1/CTF loading and direct receptor-mediated recruitment of additional transcription factors.
- Publication:
-
Science
- Pub Date:
- March 1992
- DOI:
- 10.1126/science.1347958
- Bibcode:
- 1992Sci...255.1573A