Wild-Type p53 Binds to the TATA-Binding Protein and Represses Transcription
Abstract
p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- December 1992
- DOI:
- 10.1073/pnas.89.24.12028
- Bibcode:
- 1992PNAS...8912028S