A protein kinase associated with paired helical filaments in Alzheimer disease.
Abstract
We have identified a protein kinase in immunoaffinity-purified preparations of paired helical filaments from brain tissue of individuals with Alzheimer disease. The kinase phosphorylates the filament proteins in vitro in a manner independent of second messenger regulation or of modulation by heparin and polyamines. Physiological concentrations of hemin, an oxidized heme porphyrin, inhibit the kinase and abolish Alz-50 immunoreactivity of the proteins. Since paired helical filaments are composed of hyperphosphorylated proteins, association of a protein kinase with the filaments provides a mechanism for abnormal processing of the proteins in disease.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- April 1992
- DOI:
- 10.1073/pnas.89.7.2878
- Bibcode:
- 1992PNAS...89.2878V