Human melanotransferrin (p97) has only one functional iron-binding site
Abstract
The iron-binding properties of melanotransferrin, the tumour-associated antigen also known as p97, have been investigated by UV/visible and fluorescence spectroscopy, amino acid sequence comparison, and modelling. These show that, in contrast to other transferrins, melanotransferrin binds only one Fe 3+ ion per molecule. The binding properties of its N-terminal site are similar to other transferrins, but its C-terminal site does not bind iron at all. The differences can be related to specific amino acid changes in the C-terminal site.
- Publication:
-
FEBS Letters
- Pub Date:
- January 1992
- DOI:
- 10.1016/0014-5793(92)80060-T
- Bibcode:
- 1992FEBSL.298..215B
- Keywords:
-
- Iron binding;
- Melanotransferrin;
- Transferrin;
- Melanoma cell antigen