The N-end rule is mediated by the UBC2(RAD6) ubiquitin-conjugating enzyme.
Abstract
The N-end rule relates the in vivo half-life of a protein to the identity of its amino-terminal residue. Distinct versions of the N-end rule operate in all organisms examined, from mammals to bacteria. We show that UBC2(RAD6), one of at least seven ubiquitin-conjugating enzymes in the yeast Saccharomyces cerevisiae, is essential for multiubiquitination and degradation of the N-end rule substrates. We also show that UBC2 is physically associated with UBR1, the recognition component of the N-end rule pathway. These results indicate that some of the UBC2 functions, which include DNA repair, induced mutagenesis, sporulation, and regulation of retrotransposition, are mediated by protein degradation via the N-end rule pathway.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- August 1991
- DOI:
- 10.1073/pnas.88.16.7351
- Bibcode:
- 1991PNAS...88.7351D