Molecular cloning and expresion of a protein-tyrosine phosphatase showing homology with transcription factors Fos and Jun
Abstract
A cDNA clone coding for a protein-tyrosine phosphatase (PTPase) was isolated from a rat spleen cDNA library. Nucleotide sequence of the clone showed an open reading frame coding for a polypeptide of 363 amino acids. Expression of this clone in E. coli in an expression vector showed PTPase activity. The non-catalytic region of this PTPase located at the carboxy terminus shows homology with the basic domains of transcription factors Fos and Jun. Northern blot analysis showed that a 1.7 kb transcript was present in many tissues and cells, the highest level being in macrophages. This PTPase is a rat homolog of human T-cell PTPase although it shows 3 large deletions in the carboxy terminal non-catalytic region.
- Publication:
-
FEBS Letters
- Pub Date:
- January 1991
- DOI:
- 10.1016/0014-5793(91)80205-H
- Bibcode:
- 1991FEBSL.280...65S
- Keywords:
-
- Protein-tyrosine phosphatase;
- cDNA cloning;
- Gene expression;
- Transcription factor;
- PTPase;
- Protein-tyrosine phosphatase;
- PTP-S;
- PTPase cloned from a rat spleen cDNA library