Premature termination of transcription and alternative splicing in the human transacylase (E2) gene of the branched-chain α-ketoacid dehydrogenase complex
Abstract
We have isolated a human genomic clone hgE2-14 containing exons 5,6,7 and 8 of the branched-chain α-ketoacid dehydrogenase E2 transacylase gene. Sequencing of exon B and its surrounding intronic sequences reveals complete identity with the previously reported truncated E2 cDNA (hE2-1) sequence between nucleotides 938 and 1521. We have identified consensus splice site junctions flanking exon 8 and also a cryptic 3??? splice site 370 bases upstream from the start of exon 8 in the gene. In addition, two polyadenylation signals located in the hE2-1 cDNA are also present in the intronic sequence downstream of exon 8 which promote termination of transcription. The data indicate that shortened human liver E2 transcripts undergo alternative splicing to yield mRNA of the hE2-1 type.
- Publication:
-
FEBS Letters
- Pub Date:
- January 1991
- DOI:
- 10.1016/0014-5793(91)80155-V
- Bibcode:
- 1991FEBSL.279..229L
- Keywords:
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- Genomic clone;
- cDNA;
- E2 gene;
- Alternative splicing