Translation Initiation and Ribosomal Biogenesis: Involvement of a Putative rRNA Helicase and RPL46
Abstract
Cold mutations in the SPB genes (spb1-spb7) of Saccharomyces cerevisiae suppress the inhibition of translation initiation resulting from deletion of the poly(A)-binding protein gene (PAB1). The SPB4 protein belongs to a family of adenosine triphosphate (ATP)-dependent RNA helicases. The aberrant production of 25S ribosomal RNA (rRNA) occurring in spb4-1 mutants or the deletion of SPB2 (RPL46) permits the deletion of PAB1. These data suggest that mutations affecting different steps of 60S subunit formation can allow PAB-independent translation, and they indicate that further characterization of the spb mutations could lend insight into the biogenesis of the ribosome.
- Publication:
-
Science
- Pub Date:
- March 1990
- DOI:
- 10.1126/science.2408148
- Bibcode:
- 1990Sci...247.1077S