Transforming Growth Factor β Decreases the Immunogenicity of Rat Islet Xenografts (Rat to Mouse) and Prevents Rejection in Association with Treatment of the Recipient with a Monoclonal Antibody to Interferon γ
Abstract
Culture of rat islets of Langerhans for 1 week at 37 degrees C with recombinant transforming growth factor beta prolonged the survival of islet xenografts transplanted into diabetic mouse recipients. Treatment of diabetic recipients with a neutralizing monoclonal antibody to murine interferon gamma did not affect the survival of islet xenografts cultured 7 days in control medium. However, treatment of donor islets with transforming growth factor beta in combination with treatment of diabetic recipients with interferon gamma antibody produced a 75% survival of the islet xenografts at 100 days. Fifty percent of the recipients who had accepted their graft for more than 100 days were immune unresponsive to a transplant of freshly isolated islets from the same donor strain.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- February 1990
- DOI:
- 10.1073/pnas.87.4.1591
- Bibcode:
- 1990PNAS...87.1591C