Stereochemical Course of Catalysis by the Tetrahymena Ribozyme
Abstract
The group I intron from Tetrahymena catalyzes phosphodiester transfer reactions on various RNA substrates. A modified RNA substrate with a phosphorothioate group in one stereoisomeric form at the site of reaction was synthesized in order to determine the stereochemical course of an RNA-catalyzed reaction. The reaction product was digested with a stereospecific nuclease to determine the configuration of the product phosphorothioate. The reaction occurs with inversion of configuration at phosphorus, implying an in-line pathway for the reaction.
- Publication:
-
Science
- Pub Date:
- May 1989
- DOI:
- 10.1126/science.2470151
- Bibcode:
- 1989Sci...244..692R