Production of Stable Rabbit-Mouse Hybridomas that Secrete Rabbit mAb of Defined Specificity
Abstract
Inclusion of normal rabbit serum (NRS) in culture medium after interspecific fusion of hyperimmunized rabbit spleen cells with murine SP2/0 myeloma cells produced 271 rabbit-mouse hybridomas (RMHs) that secreted rabbit immunoglobulin against group A Streptococcus (GAS). Continued use of NRS-supplemented medium during cloning yielded stabilized monoclonal RMH lines that have secreted GAS-specific rabbit antibody at concentrations similar to murine hybridomas (3 to 8 micrograms per 106 cells per 24 hours), for over 4 months of culture in vitro. The use of NRS as a medium supplement during initial culture, cloning, and stabilization of RMHs enables production of considerably more specific rabbit monoclonal antibody (mAb)--secreting RMHs than have previously been reported.
- Publication:
-
Science
- Pub Date:
- June 1988
- DOI:
- 10.1126/science.3289119
- Bibcode:
- 1988Sci...240.1788R