Quaternary tephra of Mt. Osore-zan was dated by electron spin resonance. Quartz grains were separated from the tephra and ESR signals of Al and Ti centres were measured at 77 K. The signals of Al and Ti centres which have a complicated hyperfine structure were simplified by using wider field modulations width (5 gauss) than usually used (0.5-1 gauss). The influence of 5 gauss modulation width on the measurement of the signal intensity of Al and Ti centres was investigated. The intensity of the signal increases linearly with gamma irradiation and total doses obtained were 178 to 273 Gy and ESR ages were 0.19 to 0.32 Ma. These ages agree with the estimated age of about 0.3 Ma from the correlation of volcanic ash and terrace level.