Molecular Cloning and Expression of the cDNA for the Hamster α 1-adrenergic Receptor
Abstract
The cDNA for the Syrian hamster alpha 1-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the receptor protein purified from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes a 515-residue polypeptide that shows the most sequence identity with the other adrenergic receptors and the putative protein product of the related clone G-21. Similarities with the muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells confirm that we have cloned the alpha 1-adrenergic receptor that couples to inositol phospholipid metabolism.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- October 1988
- DOI:
- 10.1073/pnas.85.19.7159
- Bibcode:
- 1988PNAS...85.7159C