Soluble factors stimulating secretory protein translocation in bacteria and yeast can substitute for each other.
Abstract
mRNA for prepro-alpha-factor (pp alpha), a yeast secretory glycoprotein, was translated in a wheat germ cell-free system that was posttranslationally supplemented either with inverted vesicles from the plasma membrane of Escherichia coli (INV) or with microsomes from Saccharomyces cerevisiae. A postribosomal supernatant (PRS) from E. coli was found to stimulate translocation of pp alpha across the INV membrane. A yeast PRS could substitute for its E. coli counterpart. Likewise, an E. coli PRS could substitute for a yeast PRS and stimulate translocation of pp alpha across yeast microsomal membranes.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- June 1987
- DOI:
- 10.1073/pnas.84.11.3723
- Bibcode:
- 1987PNAS...84.3723F