The Oncogenic Activation of Human p21ras by a Novel Mechanism
Abstract
Single amino acid changes were introduced into normal (non-oncogenic) and activated forms of the human H-ras protein at a position (residue 116) proposed on structural grounds to represent a contact site with guanine nucleotides. Substitutions at this site could significantly reduce the ability of both forms to bind and hydrolyze guanosine 5'-triphosphate; these substitutions, however, did not necessarily diminish the transforming capacity of activated derivatives. One substitution that severely impairs these functions activated the transforming potential of the otherwise normal polypeptide.
- Publication:
-
Science
- Pub Date:
- August 1986
- DOI:
- 10.1126/science.3487832
- Bibcode:
- 1986Sci...233..649W