Protein sequencing by tandem mass spectrometry.
Abstract
Methodology for determining amino acid sequences of proteins by tandem mass spectrometry is described. The approach involves enzymatic and/or chemical degradation of the protein to a collection of peptides which are then fractionated by high-performance liquid chromatography. Each fraction, containing as many as 10-15 peptides, is then analyzed directly, without further purification, by a combination of liquid secondary-ion/collision-activated dissociation mass spectrometry on a multianalyzer instrument. Interpretation of collision-activated dissociation mass spectra is described, and results are presented from a study of soluble peptides produced by treatment of apolipoprotein B with cyanogen bromide and trypsin.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- September 1986
- DOI:
- 10.1073/pnas.83.17.6233
- Bibcode:
- 1986PNAS...83.6233H