Isolation of Point Mutations in Bacteriophage Mu Attachment Regions Cloned in a λ:: Mini-Mu Phage
Abstract
Twenty-one derivatives of a lambda::mini-Mu phage containing point mutations in the Mu attachment regions were isolated after mutD mutagenesis and selection for relief from Mu-specific replicative interference of lambda growth. DNA sequence analysis revealed that the single left-end mutant had suffered a T----C transition at position 1 of the Mu sequence, while the remaining 20 right-end mutants contained single base-pair insertions or deletions within the terminal 19 base pairs. A genetic assay showed that the right-end mutations revealed by sequencing were necessary for relief of the replicative inhibition of lambda growth. The properties of these mutants suggest that the terminal 2-base-pair and subterminal 8-base-pair inverted repeats are important for Mu-specific replicative transposition.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- August 1986
- DOI:
- 10.1073/pnas.83.16.6012
- Bibcode:
- 1986PNAS...83.6012B