Deduced Primary Structure of the α Subunit of the GTP-Binding Stimulatory Protein of Adenylate Cyclase
Abstract
A bovine adrenal cDNA clone encoding the entire alpha subunit of the GTP-binding regulatory protein that stimulates adenylate cyclase (Gs) was isolated and sequenced. This cDNA directed the synthesis of the larger, 52-kDa form of the polypeptide in COS cells, even though the clone appeared to encode a 46-kDa protein. Comparison of the deduced amino acid sequence of Gs alpha with the alpha subunit of another G protein, transducin, revealed striking homologies.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- March 1986
- DOI:
- 10.1073/pnas.83.5.1251
- Bibcode:
- 1986PNAS...83.1251R