Partial molecular characterization of endothelial cell mitogens from human brain: acidic and basic fibroblast growth factors
Abstract
Two endothelial cell growth factors have been isolated from human brain extracts by a procedure involving heparin-Sepharose affinity chromatography and reverse-phase HPLC. On the basis of molecular size, amino acid composition and extended amino-terminal sequences, the two proteins are structurally closely related to bovine acidic and basic fibroblast growth factors (aFGF, bFGF). The N-terminal sequences of human and bovine aFGF diner in two positions while those for bFGF are indistinguishable. Human aFGF is microheterogenous: in addition to aFGF two N-terminally truncated forms lacking the first and the seven first amino acids, respectively, were characterized. Human and bovine mitogens possess similar mitogenic activities.
- Publication:
-
FEBS Letters
- Pub Date:
- January 1986
- DOI:
- 10.1016/0014-5793(86)80812-2
- Bibcode:
- 1986FEBSL.204..203G
- Keywords:
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- Fibroblast growth factor Heparin-Sepharose affinity chromatography HPLC Microsequencing Microheterogeneity