Nucleosome core particles suppress the thermal untwisting of core DNA and adjacent linker DNA.
Abstract
Covalently closed circular DNA is known to undergo a temperature-dependent change in helical twist. We have reconstituted nucleosome core particles onto closed circular DNA and measured the thermal untwisting of the DNA as a function of nucleosome density. The results demonstrate that the DNA associated with the nucleosome core particle does not alter its twist when the temperature is varied between 4 degrees C and 37 degrees C, and that the length of DNA prevented from thermal untwisting includes the linker as well as the core DNA.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- July 1985
- DOI:
- 10.1073/pnas.82.14.4653
- Bibcode:
- 1985PNAS...82.4653M