Insertion of DNA sequences into the human chromosomal β-globin locus by homologous recombination
Abstract
A `rescuable' plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human β-globin locus. The planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed.
- Publication:
-
Nature
- Pub Date:
- September 1985
- DOI:
- 10.1038/317230a0
- Bibcode:
- 1985Natur.317..230S