Identification and Mapping of Polypeptides Encoded by the P3HR-1 Strain of Epstein--Barr Virus
Abstract
The Epstein-Barr virus (EBV)-specified polypeptides induced upon viral replication in the P3HR-1 cell line have been examined by immunoprecipitation with a high-titer human anti-EBV serum. Twenty-five predominant polypeptides were identified in cell extracts, whereas 18 polypeptides were precipitated from cell-free translation reactions directed by total mRNA. Hybrid selection of mRNA to the BamHI DNA clones of the EBV genome and immunoprecipitation of the corresponding cell-free translation products revealed 98 EBV-specified polypeptides and their coding location along the viral genome. In addition, the viral polypeptides that bind reversibly to DNA-cellulose have been characterized and the deduced map locations of this functional group of EBV-specified polypeptides is presented.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- July 1984
- DOI:
- 10.1073/pnas.81.13.4183
- Bibcode:
- 1984PNAS...81.4183C