Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli.
Abstract
Plasmids have been constructed that direct the synthesis in Escherichia coli of heavy chains and/or light chains of an anti-carcinoembryonic antigen (CEA) antibody. Another plasmid was constructed for expression of a truncated form of heavy chain (Fd' fragment) in E. coli. Functional CEA-binding activity was obtained by in vitro reconstitution in E. coli extracts of heavy chain or Fd' fragment mixed with extracts containing light chain.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- June 1984
- DOI:
- 10.1073/pnas.81.11.3273
- Bibcode:
- 1984PNAS...81.3273C