Purification and characterization of acidic fibroblast growth factor from bovine brain.
Abstract
Acidic brain fibroblast growth factor has been purified a minimum of 35,000-fold to apparent homogeneity by a combination of differential salt precipitation, ion exchange chromatography, gel filtration, isoelectric focusing, and hydrophobic chromatography on a C4 reversed-phase HPLC column. Two microheterogeneous forms of the molecule are obtained with apparent molecular masses of 16,600 and 16,800 daltons. The mitogen is highly active with half-maximal stimulation of BALB/c 3T3 fibroblasts at about 40 pg/ml in an assay using incorporation of [methyl-3H]thymidine into DNA.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- January 1984
- DOI:
- 10.1073/pnas.81.2.357
- Bibcode:
- 1984PNAS...81..357T