Isolation of UV-resistant revertants from a xeroderma pigmentosum complementation group A cell line
Abstract
Xeroderma pigmentosum (XP) is an autosomal recessive disease. Cells cultured from XP patients are hypersensitive to the lethal effects of UV light1-6. Most XP cells are defective in an early stage in DNA repair of UV light-induced damage. The nature of the genetic defect of the XP syndrome has not been defined. To address this problem, we attempted to isolate UV-resistant cells from a cell line derived from an XP complementation group A (XPA) patient. By using a selection scheme capable of detecting one UV-resistant cell in a population of 108 cells, several UV-resistant clones were isolated at frequencies between 1 × 10-7 and 2 × 10-8. Here we describe the isolation and initial characterization of these phenotypic revertants.
- Publication:
-
Nature
- Pub Date:
- September 1984
- DOI:
- 10.1038/311390a0
- Bibcode:
- 1984Natur.311..390R