A novel method of DNA transfection by laser microbeam cell surgery
Abstract
A new technique is presented to incorporate exogeneous gene materials (DNA) into cells with a microbeam irradiation from an uv pulsed laser. A frequency-multiplied Nd:YAG laser, 355 m wavelength, 5 ns pulse duration, punches a self-healing hole of submicrometer aperture in cell membrane under selected irradiation conditions. It takes a fraction of a second for the aperture to close, long enough to allow the foreign DNA, contained in the medium, to slip into the cell. The method offers a clear advantage over existing methods: increases the success rate of DNA transfection as well as the efficiency of cell modification by orders of magnitude.
- Publication:
-
Applied Physics B: Lasers and Optics
- Pub Date:
- November 1984
- DOI:
- 10.1007/BF00697702
- Bibcode:
- 1984ApPhB..35..135T
- Keywords:
-
- PACS 07.60 - 42.80 - 87;
- 07.60;
- 42.80;
- 87