Chromophore Structure of the Physiologically Active Form (Pfr) of Phytochrome
Abstract
Chromopeptides were prepared by proteolytic digestion of phytochrome (far-red absorbing form, Pfr) and of phycocyanin. The phycocyanobilin peptide, the chromophore of which is Z,Z,Z-configurated, was modified to the Z,Z,E isomeric chromophore. It has been demonstrated earlier that the Pfr chromopeptide and the Z,Z,E-configurated phycocyanin chromopeptide behave similarly with regard to spectral and chromatographic properties and reactivity. We present evidence here, obtained by high-resolution 1H NMR spectroscopy, that both the modified phycocyanobilin chromophore and the phytochrome chromophore obtained directly from Pfr are 15E-configurated.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- October 1983
- DOI:
- 10.1073/pnas.80.20.6244
- Bibcode:
- 1983PNAS...80.6244R