Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli
Abstract
Bacterial clones containing human tissue-type plasminogen activator (t-PA) cDNA sequences were identified in a cDNA library prepared using gel-fractionated mRNA from human melanoma cells. A plasmid containing the Escherichia coli trp promoter and the cDNA sequence coding f or the 527-amino acid mature t-PA protein was constructed for expression in E. coli. A polypeptide was produced having the fibrinolytic properties characteristic of authentic human t-PA.
- Publication:
-
Nature
- Pub Date:
- January 1983
- DOI:
- 10.1038/301214a0
- Bibcode:
- 1983Natur.301..214P