High-intensity X-ray holography: An approach to high-resolution snapshot imaging of biological specimens
Abstract
The crucial physical and technological issues pertaining to the holographic imaging of biological structures with a short pulse, high intensity, high quantum energy laser were examined. The limitations of X-ray optics are discussed. Alternative holographic techniques were considered, and it was concluded that far field Fresnel transform holography (Fraunhofer holography) using a photoresist recording surface is most tractable with near term technology. The hydrodynamic expansion of inhomogeneities within the specimen is discussed. It is shown that expansion is the major source of image blurring. Analytic expressions were derived for the explosion of protein concentrations in an X-ray transparent cytoplasm, compared with numerical calculations, and corrections derived to account for the competitive transport processes by which these inhomogeneities lose energy. It is concluded that for the near term Fresnel transform holography, particularly, far field or Fraunhofer holography, is more practical than Fourier transform holography. Of the altenative fine grain recording media for use with Fresnel transform holography, a photo resist is most attractive.
- Publication:
-
NASA STI/Recon Technical Report N
- Pub Date:
- August 1982
- Bibcode:
- 1982STIN...8323581S
- Keywords:
-
- Biotechnology;
- Holography;
- Imaging Techniques;
- Microscopy;
- X Ray Imagery;
- Blurring;
- Cells (Biology);
- Fourier Transformation;
- Fresnel Diffraction;
- Hydrodynamics;
- Image Resolution;
- Photoconductors;
- Pulsed Lasers;
- Instrumentation and Photography