Signal processor for a multiparameter flow cytometer using multilaser excitation

An instrument was designed for use in a multilaser flow system to measure particles and single biological cells. Signals from as many as six input parameters can be processed through a hard wired chassis and a multichannel pulse height analyzer. If an optional microcomputer based data acquisition system is used, eight input parameters can be accepted. Signals are derived from Coulter effect electrical detectors and photodetectors measuring fluorescence and small angle light scatter along the flow stream. The versatile signal processing chassis allows selective correlation of signals for simultaneous or sequential measurements according to geometric arrangement of the lasers, optical path elements, and detectors.