An Apple 2 based image digitizing system for use with immunoelectrophoresis plates

In order to undertake basic image analysis on 2-dimensional immunoelectrophoresis plates an image digitizing system was constructed using a 48K Apple II in conjunction with a Microworks DS-65 Digisector card which takes the video signal from a television camera in a specially constructed projection box. Accuracy and reproducibility are assured by averaging up to 16 scans and normalizing to a blank plate. Careful packing of the data permits the processing of a plate on a single Apple disk while the primary disk drive carries the system software. The plate image of 70 mm by 70 mm is digitized into an array of 128 by 128 pixels each with 64 intensity levels.