A DNA-mediated transformation system for Dictyostelium discoideum.
Abstract
We have established a transformation system for Dictyostelium discoideum. The transformation vector contains the protein coding region of the Tn5 neomycin resistance gene fused to the proposed promoter of the Dictyostelium actin 8 gene; the vector also contains a sequence that acts as an autonomously replicating sequence (ars) in yeast. Using this vector, we can transform Dictyostelium vegetative amoebae to be resistant to aminoglycoside G418 at a frequency of between 10(-6) and 10(-4) of the input cells. The transformed cell lines are stable and contain vector sequences integrated within chromosomal DNA.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- December 1982
- DOI:
- 10.1073/pnas.79.23.7356
- Bibcode:
- 1982PNAS...79.7356H