Dna-Mediated Gene Transfer of β -aspartylhydroxamate Resistance into Chinese Hamster Ovary Cells
Abstract
Cell lines that have high levels of resistance to beta-aspartylhydroxamate and elevated levels of asparagine synthetase activity were selected in two steps from Chinese hamster ovary cells. Resistance to beta-aspartylhydroxmate was transferred into sensitive cells by using total genomic DNA derived from the dominant two-step mutants. The surviving colonies were characterized as transferants on the basis of transfer frequency, degree of resistance to beta-aspartylhydroxamate, increased level of asparagine synthetase activity, expression of the donor form of asparagine synthetase, codominance in hybrids, and instability of the phenotype in the absence of selection.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- September 1981
- DOI:
- 10.1073/pnas.78.9.5724
- Bibcode:
- 1981PNAS...78.5724A