Recombination between Higher Plant DNA and the Ti Plasmid of Agrobacterium tumefaciens
Abstract
The Ti plasmid sequences (T-DNA) from the octopine-producing crown gall tumor A6S/2 were isolated by molecular cloning, using the bacteriophage λ vector Charon 4A. Analysis of the cloned DNA segments indicates that the Ti plasmid sequences are covalently joined to plant nuclear DNA. These data demonstrate that genetic recombination between a eukaryote and a prokaryote can occur as a natural phenomenon.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- November 1980
- DOI:
- 10.1073/pnas.77.11.6448
- Bibcode:
- 1980PNAS...77.6448T