Continuous, clonal, insulin- and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor.
Abstract
Continuous cell lines that secrete both insulin and somatostatin were established by two cooperating groups of investiagtors from a serially transplantable, radiation-induced, rat islet cell tumor. The cell lines, named RIN-r and RIN-m, were initiated from tumors maintained in inbred rats or in athymic nude mice, respectively. The cultured cells are epithelioid, free of fibroblast contamination, and can be cloned. They have a hypodiploid chromosome number, are tumorigenic, and posses amine-handling properties, including high levels of L-dopa decarboxylase and formaldehyde-induced fluorescence. Preliminary analysis of clones revealed a spectrum of insulin secretion from undetectable to relatively high. These clonal cell lines provide important systems to study the biology of insulin and somatostatin.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- June 1980
- DOI:
- 10.1073/pnas.77.6.3519
- Bibcode:
- 1980PNAS...77.3519G