An Escherichia coli Replication Protein that Recognizes a Unique Sequence within a Hairpin Region in φ X174 DNA
Abstract
Protein n', a prepriming DNA replication enzyme of Escherichia coli, is a phi X174 DNA-dependent ATPase. Restriction of phi X174 DNA have led to the identification of a 55-nucleotide fragment that carries the protein n' recognition sequence. Molecular hybridization and sequence analysis have located this sequence within the untranslated region between genes F and G, a map location analogous to that of the unique complementary strand origin of phage G4 DNA. Within the 55-nucleotide fragment is a sequence of 44 nucleotides that forms a stable hairpin structure. This duplex may be the signal for protein n' to initiate the prepriming events that led to the start of phi X174 complementary DNA strand replication.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- February 1980
- DOI:
- 10.1073/pnas.77.2.799
- Bibcode:
- 1980PNAS...77..799S