Isolation of a yeast centromere and construction of functional small circular chromosomes
Abstract
The centromeric DNA (CEN3) from yeast chromosome III has been isolated on a 1.6 kilobase-pair segment of DNA located near the centromere-linked CDC10 locus of Saccharomyces cerevisiae. When present on a plasmid carrying a yeast chromosomal replicator, CEN3 enables that plasmid to function as a chromosome both mitotically and meiotically. Minichromosomes containing CEN3 are stable in mitosis and segregate as ordinary yeast chromosomes in the first and second meiotic divisions.
- Publication:
-
Nature
- Pub Date:
- October 1980
- DOI:
- 10.1038/287504a0
- Bibcode:
- 1980Natur.287..504C