Calibration of a microspectrofluorophotometer in absolute units of radiation
Abstract
Methods are described for direct calibration of a microspectrofluorophotometer in absolute units of radiation. The direct calibration was checked by determining the quantum yields of quinine bisulfate and rhodamine B solutions and found to give results within 7% of published values. Methods for calibrating a phosphor particle standard for day to day use are also described.
- Publication:
-
Applied Optics
- Pub Date:
- October 1980
- DOI:
- Bibcode:
- 1980ApOpt..19.3290C
- Keywords:
-
- Calibrating;
- Fluorescence;
- Microscopy;
- Spectrophotometers;
- Phosphors;
- Phototubes;
- Rhodamine;
- Transmittance;
- Instrumentation and Photography;
- FLUORESCENCE;
- MICROSCOPY;
- RADIOMETRY