DNA Restriction Endonuclease Analysis for Localization of Human β - and δ -Globin Genes on Chromosome 11
Abstract
DNA from a clone of a mouse-human hybrid that retained a human chromosome consisting of the major part of chromosome 11 and region q25-26-qter of the X chromosome was digested with various restriction endonucleases, subjected to electrophoresis in agarose gels, and transferred to nitrocellulose filters. The restriction digest pattern of the clone, when hybridized with a 32P-labeled plasmid fragment containing human beta-globin gene sequences, was a composite of the normal human and mouse (A9) patterns. When back-selected in 6-thioguanine to eliminate the 11 translocation chromosome, the hybrid cells showed only the A9 restriction pattern. These results substantiate the localization of beta- and delta-globin genes to human chromosome 11 and exclude the region 11q23-qter as the site.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- September 1979
- DOI:
- 10.1073/pnas.76.9.4563
- Bibcode:
- 1979PNAS...76.4563S