Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.
Abstract
A herpes simplex virus DNA fragment that is produced by digestion with BamHI endonuclease and carries the thymidine kinase (TK; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21) gene has been cloned in Escherichia coli. A recombinat plasmid, pFG5, has been analyzed extensively and a detailed restriction map is presented. pFG5 DNA efficiently transforms TK- mouse L cells. The TK coding sequence in the cloned fragment has been localized and a smaller recombinant plasmid, pAG0, also carrying an active TK gene, has been constructed to serve as a more convenient vector for transfer, into TK- cells, of genes previously cloned in E. coli.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- August 1979
- DOI:
- 10.1073/pnas.76.8.3755
- Bibcode:
- 1979PNAS...76.3755C