Soluble Biospecific Macromolecule for Purification of Estrogen Receptor
Abstract
An alternative to affinity chromotography purification of proteins based on the use of a water-soluble biospecific polymer has been applied to the estrogen receptor from calf uterus. The receptor (4S-trypsin form) was bound to a dextran-estradiol conjugate (molecular weight ~ 500,000) and the complex was isolated by gel filtration. Highly purified receptor was subsequently released from the dextran-estradiol conjugate by exchange with [3H]estradiol.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- July 1978
- DOI:
- 10.1073/pnas.75.7.3143
- Bibcode:
- 1978PNAS...75.3143H