Translation of melittin messenger RNA in vitro yields a product terminating with glutaminylglycine rather than with glutaminamide.
Abstract
Melittin messenger RNA from queen bee venom glands has been translated in a cell-free system from wheat germ. A product larger than promelittin is formed which has the carboxy-terminal sequence-Gln-Gln-GlyCOOH. Melittin and promelittin from venom glands terminate in -Gln-GlnCONH2. The possible role of the extra glycine residue in the formation of a COOH-terminal amide via a transamidase-like reaction is discussed.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- March 1977
- DOI:
- 10.1073/pnas.74.3.975
- Bibcode:
- 1977PNAS...74..975S