We report the successful production of V- and V0 defects in SrO. Since hydrogen has been found to be necessary for V- formation in MgO, SrO single crystals were intentionally doped with hydrogen during growth. Both these defects were detected after a short γ irradiation at 77 K in crystals previously irradiated with high doses of either neutrons, electrons, or γ rays. Electron-paramagnetic-resonance (EPR) measurements showed that the V- center exhibits < 100 > axial symmetry at 77 K characterized by g∥=2.0013(2) and g⊥=2.0705(2). At 270 K, the EPR spectrum consists of a single thermally-averaged isotropic line at g=2.047(1). An extensive electron-nuclear double-resonance search at 4.2 K revealed no charge-compensating impurities associated with this center. The V0 center also displays < 100 > axial symmetry at 77 K with the following parameters: S=1, g∥=2.0012(2), g⊥=2.0748(2), and D=127.05(5)×10-4 cm-1. The absence of V0 signals at 4.2 K established that the S=1 triplet state is an excited state. At ~ 210 K, the V0 centers begin to decay into the more stable V- center. The latter has a room-temperature half-life of ~ 25 min. Additional defects are also reported. In particular an S=I=12 axial center is tentatively identified as an (OF)2- molecular ion oriented along < 111 > directions.