In vitro transcription of chromatin in the presence of a mercurated nucleotide.
Abstract
Mercurated uridine triphosphate has been used to label transcripts of chicken reticulocyte chromatin made with Escherichia coli RNA polymerase. The mercury-labeled RNA product can be completely separated from endogenous RNA sequences in the chromatin by passage through a sulfhydryl Sepharose column. Globin cDNA hybridization to the transcript shows that only 2.6 x 10-5 of the transcript is globin RNA. In contrast to this result, erythrocte chromatin transcript contains less than one tenth as many globin RNA sequences.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- May 1976
- DOI:
- 10.1073/pnas.73.5.1564
- Bibcode:
- 1976PNAS...73.1564C