Isolation and propagation of a segment of the simian virus 40 genome containing the origin of DNA replication.
Abstract
Heteroduplex DNA molecules formed from two DNAs that differ from each other by a deletion can be cleaved at the mismatched region (a deletion loop) with the single-strand-specific S1 endonuclease. A heteroduplex DNA molecule, constructed from the DNA of simian virus 40 (SV40) mutant with a deletion of the map region 0.54-0.55 and the DNA of a second SV40 mutant having a deletion of the map segment 0.70-0.73, is cleaved twice with S1 endonuclease. One of the products is a DNA fragment of about 0.13 the length of SV40 DNA which contains the origin of SV40 DNA replication (0.67 on the SV40 DNA map).
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- May 1976
- DOI:
- 10.1073/pnas.73.5.1513
- Bibcode:
- 1976PNAS...73.1513S