Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes.
Abstract
Rabbit globin poly(A)-free and native mRNA preparations were microinjected into Xenopus oocytes. The amount of globin message remaining after incubation of injected oocytes was determined by molecular hybridization with a radioactive complementary DNA probe, synthesized by copying globin mRNA with purified RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus. The results show that 56 hr after injection about 85% of the poly(A)-free mRNA molecules are degraded, while native poly(A)-containing mRNA chains are almost completely preserved during the same period of time.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- August 1975
- DOI:
- 10.1073/pnas.72.8.3065
- Bibcode:
- 1975PNAS...72.3065M