Site-Specific Cleavage of Single-Stranded DNA by a Hemophilus Restriction Endonuclease
Abstract
Single-stranded viral DNA of bacteriophage f1 is cleaved into specific fragments by endo R\cdot HaeIII, a restriction endonuclease isolated from Hemophilus aegyptius. The sites of the single strand cleavage correspond to those of the double strand cleavage. A single-stranded DNA fragment containing only one HaeIII site is also cleaved by this enzyme. This observation suggests that the reaction of single-stranded DNA cleavage does not require the formation of a symmetrical double-stranded structure that would result from the intramolecular base-pairing between two different HaeIII sites. Other restriction endonucleases may also cleave single-stranded DNA.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- July 1975
- DOI:
- 10.1073/pnas.72.7.2555
- Bibcode:
- 1975PNAS...72.2555H