Amino-Acid Sequence of NADP-Specific Glutamate Dehydrogenase of Neurospora crassa
Abstract
A tentative primary structure of the NADP-specific glutamate dehydrogenase [L-glutamate: NADP oxidoreductase (deaminating), EC 1.4.1.4] from Neurospora crassa has been determined. The proposed sequence contains 452 amino-acid residues in each of the identical subunits of the hexameric enzyme. Comparison of the sequence with that of the bovine liver enzyme reveals considerable homology in the amino-terminal portion of the chain, including the vicinity of the reactive lysine, with only shorter stretches of homology within the carboxyl-terminal regions. The significance of this distribution of homologous regions is discussed.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- November 1974
- DOI:
- 10.1073/pnas.71.11.4361
- Bibcode:
- 1974PNAS...71.4361W