In Vitro Repair of Apurinic Sites in DNA
Abstract
Apurinic sites disappear from DNA during an incubation of the DNA with the Escherichia coli endonuclease specific for apurinic sites, DNA polymerase I (EC 2.7.7.7.), and T4 ligase (EC 6.5.1.1). Omission of any one of these three enzymes and, in particular, omission of the endonuclease specific for apurinic sites prevents this in vitro repair.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- June 1974
- DOI:
- 10.1073/pnas.71.6.2273
- Bibcode:
- 1974PNAS...71.2273V