The 3‧-Terminal Sequence of Escherichia coli 16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding Sites
Abstract
With a stepwise degradation and terminal labeling procedure the 3‧-terminal sequence of E. coli 16S ribosomal RNA is shown to be Pyd-A-C-C-U-C-C-U-U-AOH. It is suggested that this region of the RNA is able to interact with mRNA and that the 3‧-terminal U-U-AOH is involved in the termination of protein synthesis through base-pairing with terminator codons. The sequence A-C-C-U-C-C could recognize a conserved sequence found in the ribosome binding sites of various coliphage mRNAs; it may thus be involved in the formation of the mRNA.30S subunit complex.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- April 1974
- DOI:
- 10.1073/pnas.71.4.1342
- Bibcode:
- 1974PNAS...71.1342S